CENP-E falls off
نویسندگان
چکیده
منابع مشابه
BubR1 checks in with CENP-E
kinetochore protein plays both sides of the checkpoint field, according to Weaver et al. on page 551. They show that CENP-E, a mitotic checkpoint-silencing protein, also stimulates the amount of checkpoint inhibitor released from an unattached kinetochore. The mitotic checkpoint is the major mechanism preventing chromosomal loss during mammalian mitosis. Unattached kinetochores generate an inhi...
متن کاملFarnesyl transferase inhibitors block the farnesylation of CENP-E and CENP-F and alter the association of CENP-E with the microtubules.
Human tumor cell lines that are sensitive to the effects of farnesyl transferase inhibitors accumulate in G(2) --> M (except for cells with an activated Ha-ras that accumulate in G(1)). A search for CAAX box proteins from Swiss-Prot revealed more than 300 peptides. Of these, the centromeric proteins CENP-E and CENP-F are preferentially expressed during mitosis and are implicated as mediators of...
متن کاملChromosome congression is promoted by CENP-Q- and CENP-E-dependent pathways
A key step of mitosis is the congression of chromosomes to the spindle equator. Congression is driven by at least two distinct mechanisms: (1) kinetochores slide along the microtubule lattice using the plus-end directed CENP-E motor, and (2) kinetochores biorientating near the pole move to the equator through microtubule depolymerisation-coupled pulling. Here, we show that CENP-Q - a subunit of...
متن کاملCENP-E Function at Kinetochores Is Essential for Chromosome Alignment
CENP-E is a kinesin-like protein that binds to kinetochores and may provide functions that are critical for normal chromosome motility during mitosis. To directly test the in vivo function of CENP-E, we microinjected affinity-purified antibodies to block the assembly of CENP-E onto kinetochores and then examined the behavior of these chromosomes. Chromosomes lacking CENP-E at their kinetochores...
متن کاملThe mitotic kinesin CENP-E is a processive transport motor.
In vivo studies suggest that centromeric protein E (CENP-E), a kinesin-7 family member, plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. How CENP-E accomplishes this crucial task, however, is not clear. Here we present single-molecule measurements of CENP-E that demonstrate that this motor moves processively toward the plus end of microtubules, with an ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2001
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb1544iti3